Modulation of Tumour Necrosis Factor-alpha and Tumour Necrosis Factor Receptor 1 Genes in Breast Cancer Cell Lines (MCF-7) by Some Selected Indigenous Cytotoxic Plants
), Imaga NOA(2), James B(3), Makinde OE(4), Odii UO(5), Chidumem I(6), Oguntoye LA(7), Magbagbeola OA(8),
(1) Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
(2) Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
(3) Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
(4) Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
(5) Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
(6) Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
(7) Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
(8) Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
Corresponding Author
Abstract
Background: Plants have increasingly played a significant role in the treatment of cancer and infectious diseases for several
decades. Thirty percent of all cancers in women occur in the breast making it the most commonly diagnosed female cancer.
Objective: Cytotoxic properties of the 80% aqueous-ethanol crude, n-hexane, chloroform, ethylacetate, detannified, and tannin
fractions of Curculigo pilosa rhizomes, Icacina trichantha leaves, Anthocleista djalonensis leaves, Gladiolus psittacinus
bulbs, Tapinanthus bangwensis leaves, and Spilanthes ficaulis leaves were evaluated for tumour necrosis factor- (TNF-) and
tumour necrosis factor receptor (TNFR1) gene modulations.
Materials and Methods: Following brine shrimp lethality assay, fractions of each plant (detannified Icacina trichantha, crude
extract of Curculigo pilosa, hexane fraction of Spilanthes ficaulis, detannified Anthocleista djalonensis, hexane fraction of
Tapinanthus bangwensis and crude extract of Gladiolus psittacinus) with the lowest LC50 were selected for the gene expression
study. Concentrations that are five-fold lower than the LC50 of the six fractions were inoculated in triplicates into MCF-7 cells for
48 h, after which the expression of TNF- and TNFR1 genes were examined.
Results: TNFR1 gene expression was not observed in MCF-7 cell lines while TNF- expression was induced significantly
(p<0.05) by the test fractions.
Conclusion: The test fractions in this study did not induce apoptosis via the molecular mechanism of TNF- and TNFR1
expression but may support immunological activation due to the significant high levels of TNF- gene expression.
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