Plasma C-reactive Protein, Prostaglandin-F2? Metabolite and 8-Isoprostane were Elevated in Women with Dysmenorrhea

Olasore HSA(1), Oyedeji TA(2), Adebisi AA(3), James AB(4), Akinwunmi D(5), Ayoade BO(6), Olayanju SA(7), Uchefe EE(8), Babalola AR(9),


(1) Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Idi-Araba, Lagos, Nigeria.
(2) Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Idi-Araba, Lagos, Nigeria.
(3) Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Lagos State University College of Medicine, Ikeja, Lagos State, Nigeria.
(4) Department of Biochemistry and Nutrition, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria.
(5) Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Idi-Araba, Lagos, Nigeria
(6) Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Idi-Araba, Lagos, Nigeria
(7) Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Idi-Araba, Lagos, Nigeria
(8) Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Idi-Araba, Lagos, Nigeria
(9) Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Idi-Araba, Lagos, Nigeria
Corresponding Author

Abstract


Background: Dysmenorrhea is one of the most prevalent gynaecological conditions affecting the quality of life of many women
in their reproductive years. The pathomechanism is not fully understood and hence not everyone gets relief from the common
treatment approach.
Objectives: The aim of this study is to investigate the association of inflammation, uterine contraction and oxidative stress with dysmenorrhea.
Methods: Seventy young women between the ages of 17 and 33 years were recruited for the study. The participants were required to fill a questionnaire on their biodata and also fill a pain rating questionnaire on the day of their menstrual period when the pain is most severe. Five millilitres of venous blood was taken from each of them to assay for c-reactive protein (CRP), 8-isoprostane (8-IP) and 13,14-dihydro-15-keto-prostaglandin F2? which is a PGF2? metabolite (PGFM) using ELISA method.
Results: The mean ± SEM age of the participants was 20.65±0.27 years while the mean age at menarche was 12.32±0.18 years. The mean length of the cycle was 28.74±0.28 days. Over 21% of the participants reported no pain during their menstrual period while 78% reported degrees of pain ranging from mild to severe. The mean plasma CRP levels were 21.05±0.65, 24.27±1.98 and 28.30±2.11 ng/L; mean levels of 8-IP were 279.66±7.76, 309.26±10.09 and 335.10±15.86 ng/L; while the mean levels of PGFM were 2.46±0.32, 2.67±0.49 and 4.19±0.35 ng/L for those with no pain, mild/moderate and severe pain respectively. Fifteen percent of the variance in pain severity was predictable by CRP, 18% was predictable by 8-IP, while 7% was predictable by PGFM.
Conclusion: We found that inflammation, uterine contraction, and oxidative stress play significant role in the pathology of
dysmenorrhea. A combination therapy consisting of an anti-inflammatory agent and antioxidant should be considered for the
effective treatment of dysmenorrhea.

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