Detection and Characterization of Antibiotic Resistance of Gram Negative Bacteria from Hospital and Non-Hospital Environments in Benin City Nigeria

Igbinosa EO(1), Okeigbemen R(2), Beshiru A(3), Akinnibosun O(4),


(1) Applied Microbial Processes and Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154 Benin City, 300283, Nigeria. Department of Microbiology, Faculty of Life Sciences, University of Benin, PMB 1154 Benin City, 300283, Nigeria.
(2) Applied Microbial Processes and Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154 Benin City, 300283, Nigeria. Department of Microbiology, Faculty of Life Sciences, University of Benin, PMB 1154 Benin City, 300283, Nigeria.
(3) Applied Microbial Processes and Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154 Benin City, 300283, Nigeria. 3Department of Microbiology, College of Natural and Applied Sciences, Western Delta University, PMB 10 Oghara, 331101, Delta State.
(4) Applied Microbial Processes and Environmental Health Research Group, Faculty of Life Sciences, University of Benin, PMB 1154 Benin City, 300283, Nigeria. Department of Microbiology, Faculty of Life Sciences, University of Benin, PMB 1154 Benin City, 300283, Nigeria
Corresponding Author

Abstract


Background: Gram negative bacteria especially the enterobacteria are involved in clinically diagnosed infections with the global spread of antimicrobial-resistant strains. The indiscriminate discharge of untreated wastes into the environment could further increase and complicate the threat to public health.

Objective: In this study, we examined the antibiotic resistance of Gram negative bacteria from wastewater in Benin City.

Methods: A total of sixty untreated wastewater samples from hospital and non-hospital environments were collected between January and June 2019. Standard microbiological methods were employed for analysis of the samples. The antibiotic susceptibility profile of the isolates was carried out using the disc diffusion method. Biofilm formations were examined using the microtitre plate methods.

Results: The occurrence of Gram negative bacteria recovered from hospital and non-hospital sources include Citrobacter freundii 7/50 (14.0%), Klebsiella pneumoniae 9/50 (18.0%), Pseudomonas aeruginosa 6/50 (12.0%), Shigella dysenteriae 5/50 (10.0%), Escherichia coli 9/50 (18.0%), Salmonella spp. 5/50 (10.0%). All the bacterial isolates from hospital and non-hospital sources demonstrated the resistance to ampicillin 40/50 (80%), fosfomycin 31/50 (62%) and azithromycin 30/50 (60%). All 10 Pseudomonas spp., 9 E. coli and 5 Salmonella spp. from hospital and non-hospital sources formed biofilm. Extracellular enzyme formation of the isolates includes beta-haemolytic activity [hospital sources 21/25 (84%), non-hospital sources 13/25 (52%)], gelatinase production [hospital sources 16/25 (64%), non-hospital sources 14/25 (56%)].

Conclusion: This study has indicated the increasing spread of antibiotic-resistant bacteria both in clinical and non-clinical environments. Therefore, it is evident that there is need to develop effective control measures to curtail the dissemination of antibiotic resistance bacteria and properly control the discharges of wastewater into the environment.


Keywords


Detection, Characterization, Antibiotic Resistance, Gram Negative Bacteria, Hospital, Non-Hospital Environments

Full Text: PDF

Article Metrics

Abstract View : 14 times
PDF Download : 3 times

Refbacks

  • There are currently no refbacks.